| 別品名 |
Rabbit Anti-Sequestosome 1 Antibody, Phosphotyrosine-Independent Ligand For The Lck SH2 Domain Of 62 KDa, EBI3-Associated Protein Of 60 KDa, Ubiquitin-Binding Protein P62, Oxidative Stress Induced Like, EBIAP,？OSIL,？P60,？Phosphotyrosine Independent Ligand For The Lck SH2 Domain P62,？EBI3-Associated Protein P60, Paget Disease Of Bone, Sequestosome-1, FTDALS3,？NADGP, A170, DMRV, ZIP3, P62B, PDB3, ORCA, P62
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| 抗原部位 |
Internal
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Affinity Purified
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Immuno Fluorescence
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| 免疫動物 |
Rabbit
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| 交差種 |
Human
Mouse
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| GENE ID |
8878
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| Accession No.(Gene/Protein) |
NP_001135770, Q13501
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| Gene Symbol |
SQSTM1
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| 形状 |
液状
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| その他 |
[Buffer]0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemistry of Rabbit Anti SQSTM1/p62 Antibody. Tissue: Human Skeletal Muscle. Antigen Retrieval: HIER using Citrate buffer for 20 mins. Primary Antibody: Anti SQSTM1/p62 at 1:200 for 30 mins at RT. Secondary Antibody: Anti Rabbit Poly HRP IgG Ready to use for 8 min at RT. Counterstain: Hematoxylin. Result Analysis: shows intense cytoplasmic staining of myocytes consistent with that described in the Human Protein Atlas.
Immunofluorescence of Rabbit Anti-SQSTM1/p62 Antibody. Cells: HeLa cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-SQSTM1 at 5ug/mL overnight at 2-8C. Secondary Antibody: Donkey Anti-Rabbit IgG DyLightTM488 (p/n 611-741-127) at 5ug/mL for 60mins at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI, (B) Anti-SQSTM1 and DyLightTM488, (C) Merge A+B, (D) secondary only. Predicted localization: Nucleus, cytosol, lysosome, vesicle. Result Analysis: Punctate staining indicative of lysosomal/vesicle staining.
Western Blot of Rabbit Anti-SQSTM1/p62 Antibody. Lane 1: Opal Prestain Molecular Weight Marker (p/n MB-210-0500). Lane 2: Human Skeletal muscle whole tissue lysate [+]. Lane 3: U87-MG whole cell lysate [+]. Lane 4: MOLT4 whole cell lysate [-] (p/n W09-001-GK2). Lane 5: HeLa whole cell lysate [+] (p/n W09-000-364). Load: 35ug lysate per lane. Primary Antibody: Anti-SQSTM1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:40,000 for 60 mins at RT. Block: 5% BLOTTO/TBST (p/n B501-0500). Predicted MW: 38, 48 kDa. Observed MW: ~45, 60kDa. Protein undergoes acetylation, Isopeptide bond, Phosphoprotein, and Ubl conjugation.
Western Blot of Rabbit Anti-SQSTM1/p62 Antibody. Opal Prestain Molecular Weight Marker (p/n MB-210-0500). Lane 1: HEK293T whole cell lysate (p/n W09-001-GX5). Lane 2: Jurkat whole cell lysate (p/n W09-001-370). Lane 3: Mouse liver normal tissue whole tissue lysate (p/n W10-000-T020). Load: 35ug lysate per lane. Primary Antibody: Anti-SQSTM1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 60 mins at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: 38, 48 kDa. Observed MW: ~38, 47, 60kDa. Protein undergoes acetylation, Isopeptide bond, Phosphoprotein, and Ubl conjugation.
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Immunohistochemistry of Rabbit Anti SQSTM1/p62 Antibody. Tissue: Human Skeletal Muscle. Antigen Retrieval: HIER using Citrate buffer for 20 mins. Primary Antibody: Anti SQSTM1/p62 at 1:200 for 30 mins at RT. Secondary Antibody: Anti Rabbit Poly HRP IgG Ready to use for 8 min at RT. Counterstain: Hematoxylin. Result Analysis: shows intense cytoplasmic staining of myocytes consistent with that described in the Human Protein Atlas.
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| メーカー |
品番 |
包装 |
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RKL
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600-401-HB8S
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25 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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