別品名 |
Rabbit Anti-HEY1 Antibody, BHLHB31, CHF2, HERP2, HESR1, HRT1, Hairy/Enhancer-Of-Split Related With YRPW Motif 1, Hes Related Family BHLH Transcription Factor With YRPW Motif 1, Hairy And Enhancer Of Split-Related Protein 1, Class B Basic Helix-Loop-Helix Protein 31, Cardiovascular Helix-Loop-Helix Factor 2, Hairy-Related Transcription Factor 1, HES-Related Repressor Protein 1, HRT-1, HESR-1, CHF-2, OAF1
|
抗原部位 |
Internal
|
種由来 |
Human
|
標識物 |
Unlabeled
|
精製度 |
Affinity Purified
|
適用 |
Western Blot Enzyme Linked Immunosorbent Assay Immunohistochemistry
|
免疫動物 |
Rabbit
|
交差種 |
Human Mouse
|
GENE ID |
23462
|
Accession No.(Gene/Protein) |
NP_001035798, Q9Y5J3
|
Gene Symbol |
HEY1
|
形状 |
液状
|
その他 |
[Buffer]0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
|
参考文献 |
Kathiriya et al. (2004). Hairy-related Transcription Factors Inhibit GATA-dependent Cardiac Gene Expression through a Signal-responsive Mechanism. Journal of Biological Chemistry, 279(52), 54937-54943. doi:10.1074/jbc.m409879200
|
[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
|
※サムネイル画像をクリックすると拡大画像が表示されます。
Immunohistochemistry of Rabbit Anti HEY1 Antibody. Tissue: Human Cerebellum. Fixative: none. Antigen Retrieval: HIER using Citrate buffer for 20 minutes. Primary Antibody: Anti HEY1 at 1:200 for 30 minutes at RT. Secondary Antibody: Ready to Use Anti Rabbit IgG HRP for 8 minutes at RT. Counterstain: Hematoxylin. Results: HEY1 showed diffuse cytoplasmic/membranous staining of low to moderate intensity of cells in the molecular layer, Pukinje cells layer, and granular cell layer in human cerebellum.
Western Blot of Rabbit Anti-HEY1 Antibody. Lane 1: Opal PreStained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HEY1 variant 2 human recombinant protein (50ng) [+]. Lane 3: A-172 lysate (p/n W09-001-GL5) (35ug) [+]. Lane 4: Jurkat lysate (p/n W09-001-370) (35ug) [+]. Lane 5: Mouse brain lysate (p/n W10-000-T004) (35ug) [+]. Primary Antibody: Anti-HEY1 at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG [H&L] Mx10 HRP (p/n 611-103-122) at 1:70,000 for 30 minutes at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: ~37kDa recombinant, ~33kDa endogenous. Observed: ~35-38kDa. Exposure: 19 sec.
|
|
Immunohistochemistry of Rabbit Anti HEY1 Antibody. Tissue: Human Cerebellum. Fixative: none. Antigen Retrieval: HIER using Citrate buffer for 20 minutes. Primary Antibody: Anti HEY1 at 1:200 for 30 minutes at RT. Secondary Antibody: Ready to Use Anti Rabbit IgG HRP for 8 minutes at RT. Counterstain: Hematoxylin. Results: HEY1 showed diffuse cytoplasmic/membranous staining of low to moderate intensity of cells in the molecular layer, Pukinje cells layer, and granular cell layer in human cerebellum.
|
|
|
メーカー |
品番 |
包装 |
RKL
|
600-401-HB5
|
100 UG
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
保存温度 |
-20℃
|
|