Anti GAPDH  

Immunofluorescence Microscopy of Rabbit Anti-Glyceraldehyde 3-Phosphate Dehydrogenase Antibody. Cells: HeLa cells. Fixation: Ice Cold Methanol. Permeabilization: Ice Cold Methanol. Primary antibody: GAPDH antibody at 1:250 overnight at 4°C. Secondary antibody: Goat Anti-Rabbit DyLight？488 (p/n 611-141-122) at 1:1,000 for 1hr at RT. Localization: GAPDH is cytoplasmic. Staining: (A) Anti-GAPDH (green), (B) Hoechst Nuclear stain (blue), (C) merge, (D) secondary only and Hoechst Nuclear stain.Magnification Objective: 20x.

Western Blot of Rabbit anti-GAPDH antibody. Lane 1: Opal Pre-stained ladder (p/n MB-210-0500). Lane 2: NIH/3T3 Lysate (p/n W10-000-358). Lane 3: HEK293 lysate (p/n W09-000-365). Lane 4: MOLT-4 Lysate (p/n W09-001-GK2). Lane 5: A549 Lysate (p/n W09-001-372). Lane 6: HeLa Lysate (p/n W09-000-364). Lane 7: NIH 3T3 Nuclear Lysate (W10-001-A74). Lane 8: HeLa Nuclear Lysate (p/n W09-001-367). Lane 9: Jurkat Lysate (p/n W09-001-370). Lane 10: Opal Pre-stained ladder (p/n MB-210-0500). Load: 35 μg per lane. Primary antibody: GAPDH antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 36 kDa for GAPDH.