Anti BCL3

Immunofluorescence Microscopy of Rabbit anti BCL3 antibody. Tissue: U 87 MG cells. Fixation: 4% PFA. Antigen retrieval: not required. Primary antibody: BCL3 antibody at 1 ug/mL overnight at 4C. Secondary antibody: Donkey Anti Rabbit IgG DyLightTM 488 (p/n 611 741 127) at 5 ug/ml for 2 h at RT. Localization: BCL3 is subcellularly localized in the cytosol, nucleoplasm, midbody and vesicles. Staining: BCL3 as green fluorescent signal with DAPI (blue) nuclear counterstain.

Immunofluorescence microscopy of BCL3 in Caco-2 cells using FITC-conjugated Fluorescent TrueBlotR anti-rabbit IgG for detection. Caco-2 cells were fixed with 4% PFA, blocked (5% mouse serum/.3% Triton X-1 in 1X PBS ) for 1 hr, then incubated with 15 ug/mL of anti-BCL3 primary antibody (Cat. No. 6-41-GU4) at 4C overnight. Following 3 washes in 1X PBS for 5 min each, 5 ug/mL of FITC-conjugated Fluorescent TrueBlotR anti-rabbit IgG was added and allowed to incubate for 1 hr at room temperature. Nuclei were counterstained with DAPI present in mounting medium. The predicted main localization is nucleoplasm. Additional localization in some cell types includes vesicles and midbody. (a) BCL3 (b) DAPI (c) merged DAPI/BCL3 (d) secondary antibody only. Image taken at 4X magnification.

Western Blot of Rabbit anti-BCL3 antibody. Lane 1: MW ladder (p/n MB-21-5). Lane 2: HEK293T WCL. Lane 3: BCL3 overexpressing HEK293T lysate. Load: 1 ug per lane. Primary antibody: BCL3 antibody at 1:1 for overnight at 4C. Secondary antibody: goat anti-rabbit secondary Antibody DyLightTM 488 antibody (p/n 611-141-122) at 1:2, for one hour at RT. Block: BlockOut blocking buffer (p/n MB-73) one hour at RT. Predicted/Observed size: 48 kda. Other band(s): BCL3 processing caused by ubiquitination, phosphorylations.

Western Blot of Rabbit anti-BCL3 antibody. Lane 1: MW ladder (p/n MB-21-5). Lane 2: U-87 MG WCL. Lane 3: C2C12 WCL (p/n W1-1-GL7). Lane 4: NIH/3T3 WCL (p/n W1--358). Load: 1 ug per lane. Primary antibody: BCL3 antibody at 1:1 for overnight at 4C. Secondary antibody: goat anti-rabbit secondary HRP antibody (p/n 611-13-122) at 1:7, for one hour at RT. Block: 1% Casein (p/n MB-81-1) (PVDF) blocking buffer one hour at RT. Predicted/Observed size: 47 kda. Other band(s): BCL3 processing caused by ubiquitination, phosphorylations.