Anti xCT  

Immunofluorescence Microscopy of Rabbit anti-xCT antibody. Tissue: PC3 cells. Fixation: 100% MeOH. Antigen retrieval: not required. Primary antibody: xCT antibody at 10 μg/mL overnight at 4？C. Secondary antibody: Donkey Anti-Rabbit IgG DyLight？ 488 (p/n 611-741-127) at 5 ug/ml for 2 h at RT. Localization: xCT is localized on the cell membrane and vesicles. Staining: xCT as green fluorescent signal with DAPI (blue) nuclear counterstain.

Western Blot of Rabbit anti-xCT antibody. Lane 1: A549 WCL (p/n W09-001-372). Lane 2: HCT-116 WCL (p/n W09-001-GM4). Lane 3: HeLa WCL (p/n W09-000-364). Load: 10 μg per lane. Primary antibody: xCT antibody at 1:1000 for overnight at 4°C. Secondary antibody: donkey anti-rabbit secondary DyLight？488 antibody (p/n 611-741-127) at 1:20,000 for one hour at RT. Block: BlockOut blocking buffer (p/n MB-073) one hour at RT. Predicted/Observed size: 56 kda. Other band(s): xCT processing caused by dimerization, glycosylation, and/or phosphorylation.

Flow Cytometry of rabbit anti-xCT antibody. Cells: breast carcinoma cells Primary antibody: xCT antibody at 1.0 μg/mL for one hour at 4°C. Secondary antibody: Donkey anti-Rabbit IgG Dylight？488 Antibody p/n (611-741-127) at 1 ug/ml in 200 ul for one hour on ice.

Western Blot of Rabbit anti-xCT antibody. Lane 1: recombinant BCL3 (unrelated negative control). Lane 2: NIH 3T3 WCL (p/n W10-000-358). Lane 3: A549 WCL (p/n W09-001-372). Load: 10 μg per lane. Primary antibody: xCT antibody at 1:1000 for overnight at 4°C. Secondary antibody: goat anti-rabbit secondary HRP antibody (p/n 611-103-122) at 1:20,000 for one hour at RT. Block: 5% BSA blocking buffer one hour at RT. Predicted/Observed size: 56 kda. Other band(s): xCT processing caused by dimerization, glycosylation, and/or phosphorylation.