Anti KLF4

Immunofluorescence Microscopy of Rabbit anti KLF4 antibody. Tissue: PC 3. Fixation: 0.5% PFA [A,C]. 0.5% MeOH [B, D]. Antigen retrieval: not required. Primary antibody: KLF4 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Anti RABBIT IgG DyLightTM 488 Conjugated Preadsorbed (p/n 611 741 127) at 5 ug/ml for 1 h at RT. Localization: cytoplasmic and nuclear Staining: Target as green fluorescent signal with DAPI (blue) nuclear counterstain.

Western Blot of Rabbit anti-KLF4 antibody. Lane 1: MW ladder (opal pre-stained) p/n (MB-210-0500). Lane 2: U-87 WCL (p/n W09-001-GX2). Lane 3: C2C12 WCL (p/n W10-001-GL2). Lane 4: NIH/3T3 WCL (p/n W10-000-358). Lane 5: THP-1 WCL (p/n W09-001-GX1). Lane 6: J774A.1 WCL (p/n W10-001-GX3). Lane 7: PC-3 WCL (p/n W09-001-GV6). Lane 8: HT-29 WCL (p/n W09-001-BS0). Lane 9: Mouse colon (W10-000-GZ1). Lane 10: A549 WCL (p/n W09-001-372). Lane 11: MOLT-4 WCL (p/n W09-001-GK2). Lane 12: HeLa WCL (p/n W09-001-364). Lane 13: MW ladder (opal pre-stained) p/n (MB-210-0500). Load: 10 μg per lane. Primary antibody: KLF4 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n 611-103-122) at 1:70,000 for 1 hr at RT. Block: 5% BLOTTO (p/n B501-0500) 30 min at RT. Predicted/Observed size: ~55 kDa for KLF4.

Western Blot of Rabbit Anti-KLF4 Antibody. Lane 1: Opal Prestained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: NCCIT Whole Cell Lysate. Load: 30ug lysate. Primary Antibody: Rabbit Anti-KLF4 at 1ug/mL overnight at 4C. Secondary Antibody: Goat Anti-Rabbit HRP Antibody (p/n 611-103-122) at 1:10,000 at RT for 1hr. Block: BLOTTO (p/n B501-0500). Predicted/Observed size: ~55 kDa for KLF4.