Anti MEK2  

Western Blot of Anti-MEK2 C-term Antibody. Lane 1: MEK1 rec lysate. Lane 2: MEK2 rec lysate. Load: 10ug. Primary Antibody: Anti-MEK2 at 1μg/mL overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit Peroxidase Conjugated Antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Blocking: BlockOut Universal Blocking buffer (p/n MB-073). Predicted MW: 45kDa.

PAGE-MAP (microsphere affinity proteomics) of Rabbit Anti-MEK2/MAP2K2 Antibody (Catalog Number: 600-401-GQ2, Lot Number: 36409). Antibody array western blot binding of gelfree size separated fractions of multiple lysates (solid lines) and shotgun mass spectroscopy identification (dashed lines) of the target band run in parallel correlate confirming the specificity of this antibody against MEK2/MAP2K2. Data was provided by the Lund-Johansen lab of Oslo University Hospital. For more information on PAGE-MAP/IP-MS identification of antibody specificity and its large-scale implementation for antibody validation see Sikorski et. al., (2018) Nature Methods 15, 909-912.

Immunohistochemistry with anti-MEK2 (N-Term) antibody showing positive staining in human glioblastoma tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-MEK2 (N-Term) antibody 600-401-GN8 at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.