Anti MEK1  

Western Blot of Anti-MEK1 Antibody. Lane 1: Molecular Weight Marker. Lane 2: MEK1 recombinant lysate. Lane 3: MEK2 recombinant lysate. Load: 10μg. Primary Antibody: Anti-MEK1 (c-term) at 1μg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit peroxidase conjugated antibody at 1:40,000 for 30 minutes RT. Blocking: BlockOut Universal Blocking buffer (p/n MB-073). Predicted Size: 43.5kDa.

Western Blot of Rabbit anti-MEK1 antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: A549 Lysate (p/n W09-001-372). Lane 7: LNCap Lysate (p/n W09-001-GJ9). Lane 8: MOLT-4 Lysate (p/n W09-001-GK2). Lane 9: Ramos Lysate (p/n W09-000-GK4). Lane 10: Raji Lysate (p/n W09-001-368). Lane 11: A-172 Lysate (p/n W09-001-GL5). Lane 12: NIH/3T3 Lysate (p/n W10-000-358). Load: 35 μg per lane. Primary antibody: MEK1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n B501-0500) for 30 min at RT. Predicted/Observed size: 43kDa for MEK1.

Immunohistochemistry with anti-MEK1 (C-Term) antibody showing positive staining in human glioblastoma tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-MEK1 (C-Term) antibody 600-401-GQ0 at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.