Anti PDL1  

Immunofluorescence Microscopy of Goat anti-PDL1 antibody. Cell line: MDA-MB-231 (p/n W09-001-GK6). Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: PLD1 antibody at 10 μg/mL for overnight at 4°C. Secondary antibody: Anti-Goat IgG DyLight？488 (Rabbit) Conjugated Preadsorbed (p/n 605-441-013) at 5 ug/ml for 2 hrs at RT. Localization: PDL1 is located in the cell membrane and cytoplasm. Staining: PDL1 as green fluorescent signal with DAPI (blue) nuclear counterstain.

Western Blot of Goat anti-PDL1 antibody. Lane 1: Molecular Weight. Lane 2: r PDL1 Recombinant protein. Load: 2μg per lane. Primary antibody: PDL1 antibody at 5 ug/mL for overnight at 4°C. Secondary antibody: Dky-a-Gt IgG HRP (p/n 605-703-125) and Rb-a-Gt IgG DL488 (p/n 605-441-013) at 1:40,000 for 45 min at RT. Block: 5% w/v BSA, 1X TBS, 0.1% TweenR 20 overnight at 4°C. Predicted/Observed size: ~33 kda for PDL1. Other band(s): none.

Western Blot of Goat anti-PDL-1 antibody. Lane 1: MW ladder opal pre-stained (p/n MB-210-0500). Lane 2: U-251 WCL (p/n W09-001-GY4). Lane 3: MDA-MB-231 WCL (p/n W09-001-GK6). Lane 4: Ms Heart lysate (p/n W10-000-T014). Lane 5: recombinant human PDL-1 protein. Lane 6: A549 WCL (p/n W09-001-372). Lane 7: A549 IFNy Stimulated WCL (p/n W09-001-GY5). Load: Lysates 10μg per lane, protein at 5.0ng per lane. Primary antibody: PDL-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: goat secondary HRP antibody (p/n 611-103-122) at 1:40,000 for 45 min at RT. Block: BlockOut (p/n MB-073) overnight at 4°C. Predicted/Observed size: 33 kDa for PDL-1.