| 別品名 |
Chicken Anti-X-Ray Repair Cross Complementing 1 Antibody, X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 1, X-Ray Repair Cross-Complementing Protein 1, DNA Repair Protein XRCC1, SCAR26, RCC
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| 抗原部位 |
N-Terminus
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Purified
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| 適用 |
Western Blot
Immuno Fluorescence
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| 免疫動物 |
Chicken
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| 交差種 |
Human
Mouse
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| GENE ID |
7515
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| Accession No.(Gene/Protein) |
NP_006288, P18887
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| Gene Symbol |
XRCC1
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| 形状 |
液状
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| その他 |
[Buffer]0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Immunofluorescence of Chicken Anti XRCC1 Antibody. Cells: U2OS cells. Fixative: 100% MeOH. Permeabilization: 0.3% Triton X 100. Primary Antibody: Anti XRCC1 at 10ug/mL overnight at 2 8C. Secondary Antibody: Donkey Anti Chicken IgG DyLightTM488 (p/n 603 141 126) at 5ug/mL for 60mins at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI, (B) Anti XRCC1 and DyLightTM488, (C) Merge A+B, (D) secondary only. Expected Localization: Nucleus and Nucleoplasm.
Western Blot of Chicken Anti-XRCC1 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: U-2OS whole cell lysate [+] (p/n W09-001-374). Lane 3: MOLT-4 whole cell lysate [+] (p/n W09-001-GK2). Lane 4: Human testis whole tissue lysate [+]. Lane 5: Mouse liver, adult, whole tissue lysate [-] (p/n W10-000-T020). Load: 35μg/lane. Primary Antibody: Anti-XRCC1 at 5μg/mL overnight at 2-8°C. Secondary Antibody: Goat anti-Chicken IgG HRP (p/n 603-103-002) at 1:40,000 for 60min at RT. Expected MW: ~70kDa, Isopeptide bond, Phosphoprotein. Observed MW: at arrow ~90kDa. Consistent with other commercial antibodies detecting bands at ~80-95kDa.
Western Blot of Chicken Anti-XRCC1 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Marker (p/n MB-210-0500). Lane 2: XRCC1 overexpressing HEK293T lysate [+] (10ug). Lane 3: HEK293T lysate [+] (W09-001-GX5). Lane 4: Jurkat whole cell lysate [+] (W09-001-370). Lane 5: NIH-3T3 whole cell lysate [+] (W10-000-358). Load: 10ug/lane. Primary Antibody: Anti-XRCC1 at 1:500 overnight at 2-8C. Secondary Antibody: Goat anti-Chicken IgG HRP (p/n 603-103-002) at 1:40,000 for 60min at RT. Expected MW: ~70kDa, Isopeptide bond, Phosphoprotein. Observed MW: ~70, ~80kDa arrow.
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Immunofluorescence of Chicken Anti XRCC1 Antibody. Cells: U2OS cells. Fixative: 100% MeOH. Permeabilization: 0.3% Triton X 100. Primary Antibody: Anti XRCC1 at 10ug/mL overnight at 2 8C. Secondary Antibody: Donkey Anti Chicken IgG DyLightTM488 (p/n 603 141 126) at 5ug/mL for 60mins at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI, (B) Anti XRCC1 and DyLightTM488, (C) Merge A+B, (D) secondary only. Expected Localization: Nucleus and Nucleoplasm.
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| メーカー |
品番 |
包装 |
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RKL
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200-901-MG5S
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25 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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