Anti ERK2  

Western Blot of Mouse anti-ERK2 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: ERK1 50ng. Lane 3: ERK2 50ng. Primary Antibody: Anti-ERK2 (internal) Monoclonal Antibody at 1μg/mL. Secondary Antibody: Rabbit anti-Mouse IgG HRP (p/n 610-403-C46) at 1:40,000. Blocking Buffer: BlockOut (p/n MB-073) for 30 min at RT. Expect: ~41kDa seen in lane 3 only.

ELISA results of purified Mouse Anti-ERK2 (internal) Monoclonal Antibody tested against BSA-conjugated peptide of immunizing peptide ERK2 internal sequence (green line), as well as ERK2 c-term, ERK1 c-term, and ERK1 n-term. Each well was coated in duplicate with 0.1μg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Rb-Anti-Ms IgG HRP conjugate (p/n 610-403-C46), and TMB substrate (p/n TMBE-1000).