| 別品名 |
mouse anti-ERK2 antibody, MAPK1, ERK, ERK-2, ERK2, MAPK2, P42MAPK, PRKM1, PRKM2, ERK-2 Antibody
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| 抗原部位 |
Internal
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| 種由来 |
Human
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| 標識物 |
Unlabeled
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| 精製度 |
Ig fraction - Protein A
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 免疫動物 |
Mouse
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| クローン |
3H9.A1.A8
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| 交差種 |
Human
Mouse
Rat
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| Accession No.(Gene/Protein) |
P28482
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| Gene Symbol |
MAPK1
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| 形状 |
液状
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| その他 |
[Buffer]0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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| 参考文献 |
Roads to melanoma: Key pathways and emerging players in melanoma progression and oncogenic signaling. Biochim Biophys Acta. 2016 Feb 1;1863(4):770-784. doi: 10.1016
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
ELISA results of purified Mouse Anti ERK2 (internal) Monoclonal Antibody tested against BSA conjugated peptide of immunizing peptide ERK2 internal sequence (green line), as well as ERK2 c term, ERK1 c term, and ERK1 n term. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5μg/ml and the X axis represents the Log10 of a 3 fold dilution. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB 060 1000), Rb Anti Ms IgG HRP conjugate (p/n 610 403 C46), and TMB substrate (p/n TMBE 1000).
Western Blot of Mouse anti-ERK2 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: ERK1 50ng. Lane 3: ERK2 50ng. Primary Antibody: Anti-ERK2 (internal) Monoclonal Antibody at 1ug/mL. Secondary Antibody: Rabbit anti-Mouse IgG HRP (p/n 610-403-C46) at 1:40,000. Blocking Buffer: BlockOut (p/n MB-073) for 30 min at RT. Expect: ~41kDa seen in lane 3 only.
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ELISA results of purified Mouse Anti ERK2 (internal) Monoclonal Antibody tested against BSA conjugated peptide of immunizing peptide ERK2 internal sequence (green line), as well as ERK2 c term, ERK1 c term, and ERK1 n term. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5μg/ml and the X axis represents the Log10 of a 3 fold dilution. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB 060 1000), Rb Anti Ms IgG HRP conjugate (p/n 610 403 C46), and TMB substrate (p/n TMBE 1000).
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| メーカー |
品番 |
包装 |
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RKL
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200-301-GQ1S
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25 UL
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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