Anti c-Kit / CD117, phospho Tyr703  

1/100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22ﾂｰC. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.

1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22ﾂｰC. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

1:200 staining rat brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22ﾂｰC. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

1:200 staining rat kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22ﾂｰC. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

1:200 staining rat kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22ﾂｰC. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

1:200 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22ﾂｰC. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22ﾂｰC. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Staining H526 cells treated with SCF cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37ﾂｰC. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37ﾂｰC. A Alexa Fluorﾂｮ 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.

Western blot analysis of KIT phosphorylation expression in EGF treated HepG2 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.

Western blot analysis of Phospho-KIT (Tyr703) expression in various lysates