Anti IgG1

ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG1, Human ads-BIOT (SB Cat. No. 1070-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

Frozen human lung tissue section was stained with anti-DC-SIGN followed by an AP conjugated secondary antibody and New Fucshin. The section was then stained with anti-CD207 followed by Goat Anti-Mouse IgG1, Human ads-BIOT (SB Cat. No. 1070-08), AP conjugated streptavidin, and Blue AP.Image from Van Pottelberge GR, Bracke KR, Demedts IK, De Rijck K, Reinartz SM, van Drunen CM, et al. Selective accumulation of langerhans-type dendritic cells in small airways of patients with COPD. Respir Res. 2010;11:35. Figure 2(a)

Human dermal fibroblasts stimulated with unstimulated macrophages for 72 h were stained with anti-collagen type I followed by Goat Anti-Mouse IgG1, Human ads-BIOT (SB Cat. No. 1070-08), CY3 conjugated Streptavidin, and DAPI.Image from Ploeger DT, Hosper NA, Schipper M, Koerts JA, de Rond S, Bank RA. Cell plasticity in wound healing: paracrine factors of M1/ M2 polarized macrophages influence the phenotypical state of dermal fibroblasts. Cell Commun Signal. 2013;11:29. Figure 7(b)

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat Anti-Mouse IgG1, Human ads-BIOT (SB Cat. No. 1070-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).