Anti IgG1

ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG1, Human ads-HRP (SB Cat. No. 1070-05).

Total cell lysates from HL60 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Bax-UNLB (SB Cat. No. 10055-01). Proteins were visualized using Goat Anti-Mouse IgG1, Human ads-HRP (SB Cat. No. 1070-05) secondary antibody and chemiluminescent detection.

Paraffin embedded explanted islet slab was stained with anti-insulin followed by Goat Anti-Mouse IgG1, Human ads-HRP (SB Cat. No. 1070-05) and developed with DAB.Image from Neufeld T, Ludwig B, Barkai U, Weir GC, Colton CK, Evron Y, et al. The efficacy of an immunoisolating membrane system for islet xenotransplantation in minipigs. PLoS One. 2013;8(8):e70150. Figure 4(d)

Lane 1 - DEC Monoclonal AntibodyLane 2 - DEC-HER2 Monoclonal AntibodyLane 3 - Control Ig-HER2 Monoclonal AntibodyHER2 extracellular domain was cloned in frame into the COOH terminus of anti-DEC (DEC-HER2). DEC, DEC-HER2, and control mouse IgG were resolved by electrophoresis, transferred to membrane, and probed with Goat Anti-Mouse IgG1, Human ads-HRP (SB Cat. No. 1070-05) and chemiluminescent detection.Image from Wang B, Zaidi N, He L, Zhang L, Kuroiwa JM, Keler T, et al. Targeting of the non-mutated tumor antigen HER2/neu to mature dendritic cells induces an integrated immune response that protects against breast cancer in mice. Breast Cancer Res. 2012;14:R39. Figure 1(b)