Anti IgG

ELISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05).

Total cell lysates from NIH/3T3 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bax-UNLB (SB Cat. No. 10060-01). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.

Human IgG-UNLB (SB Cat. No. 0150-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9040-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.

Tissue lysate from rTgFKBP5 double transgenic mouse hippocampus was pre-cleared with Mouse IgG-UNLB (SB Cat. No. 0107-01) and immunoprecipitated with anti-GluR1 or Rabbit IgG-UNLB (SB Cat. No. 0111-01). Protein was resolved by electrophoresis, transferred to membrane, and probed with anti-GluR1 or anti-HSP90 followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08), Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05), an HRP conjugated biotin binding protein, and chemiluminescent detection.Image from Blair LJ, Criado-Marrero M, Zheng D, Wang X, Kamath S, Nordhues BA, et al. The disease-associated chaperone FKBP51 impairs cognitive function by accelerating AMPA receptor recycling. eNeuro. 2019;6:e0242-18.2019. Figure 8(c)