IgG, Isotype Control  

Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.

Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit IgG-UNLB isotype control (SB Cat. No. 0111-01; left) and Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-BIOT (SB Cat. No. 6440-08, Streptavidin-CY3 (SB Cat. No. 7100-12), and DAPI.

Paraffin embedded human gastric cancer tissue was stained with Rabbit IgG-UNLB isotype control (SB Cat. No. 0111-01; left) and Rabbit Anti-Human IgG(H+L), Mouse ads-UNLB (SB Cat. No. 6145-01; right) followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-AF555 (SB Cat. No. 6440-32) and DAPI.

Lane 1 - 1 mg Rabbit IgGLane 2 - 0.5 mg Rabbit IgGRabbit IgG (SB Cat. No. 0111-01) was resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit IgG-AP (SB Cat. No. 4090-04). Proteins were visualized using BCIP/NBT One Component Membrane Substrate (Blue), Solution (SB Cat. No. 0430-01).

ELISA plate was coated with serially diluted Rabbit IgG-UNLB (SB Cat. No. 0111-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.

Tissue lysate from rTgFKBP5 double transgenic mouse hippocampus was pre-cleared with Mouse IgG-UNLB (SB Cat. No. 0107-01) and immunoprecipitated with anti-GluR1 or Rabbit IgG-UNLB (SB Cat. No. 0111-01). Protein was resolved by electrophoresis, transferred to membrane, and probed with anti-GluR1 or anti-HSP90 followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08), Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05), an HRP conjugated biotin binding protein, and chemiluminescent detection.Image from Blair LJ, Criado-Marrero M, Zheng D, Wang X, Kamath S, Nordhues BA, et al. The disease-associated chaperone FKBP51 impairs cognitive function by accelerating AMPA receptor recycling. eNeuro. 2019;6:e0242-18.2019. Figure 8(c)

Frozen mouse spinal cord sections were stained with anti-BAFF-R (right) and Rabbit IgG-UNLB (SB Cat No. 0111-01; left) followed by a CY5 conjugated secondary antibody.Images from Tada S, Yasui T, Nakatsuji Y, Okuno T, Koda T, Mochizuki H, et al. BAFF controls neural cell survival through BAFF receptor. PLoS One. 2013;8(7):e70924. Figure 1(c/d)

Human T cell leukemia cell line Jurkat was stained with Rabbit IgG-UNLB (SB Cat. No. 0111-01; gray) and Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01) followed by Mouse Anti-Rabbit IgG-PE (SB Cat. No. 4090-09).

Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit Light Chain-HRP (SB Cat. No. 4060-05) followed by chemiluminescent detection.

Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) followed by Streptavidin-HRP (SB Cat. No. 7105-05) and chemiluminescent detection.