IgG, Isotype Control  

ELISA plate was coated with serially diluted Mouse IgG-UNLB (SB Cat. No. 0107-01). Immunoglobulin was detected with Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.

Tissue lysate from rTgFKBP5 double transgenic mouse hippocampus was pre-cleared with Mouse IgG-UNLB (SB Cat. No. 0107-01) and immunoprecipitated with anti-GluR1 or Rabbit IgG-UNLB (SB Cat. No. 0111-01). Protein was resolved by electrophoresis, transferred to membrane, and probed with anti-GluR1 or anti-HSP90 followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08), Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05), an HRP conjugated biotin binding protein, and chemiluminescent detection.Image from Blair LJ, Criado-Marrero M, Zheng D, Wang X, Kamath S, Nordhues BA, et al. The disease-associated chaperone FKBP51 impairs cognitive function by accelerating AMPA receptor recycling. eNeuro. 2019;6:e0242-18.2019. Figure 8(c)

Human peripheral blood lymphocytes were stained with Mouse IgG-UNLB (SB Cat. No. 0107-01) followed by Goat Anti-Mouse IgG, Human ads-FITC (SB Cat. No. 1030-02).