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Immunohistochemistry of Mouse Anti-MUC4 Antibody. Tissue/Cell: A) hu Mia PACA2 [-]. B) hu Capan2 pancreatic ductal adenocarcinoma cell line [+]. C) hu HPAFII cell pellet [+]. D) Control mouse liver [-]. E) hu Control PANC1 cell pellet [-]. F) hu HPAC cell pellet [+]. Tissues: FFPE containing normal and malignant pancreatic tissues. Cell pellets: Appr. 10 million cells were collected from flasks without enzymatic treatment and centrifuged; thrombin and Fibrinogen was added. The resulting pellet of clotted cells was collected. Fixative: 10% neutral buffered formalin at RT. Antigen Retrieval: HIER citrate buffer for 20 min. Primary Antibody: Anti-MUC4 Antibody at 1:1000 in A, B, C, F. Isotype control reagent used in negative control D, E. Secondary Antibody: Anti-Mouse IgG. Counter Stain: Bond Polymer Refine Detection Kit. Analysis Results: No staining was observed for control liver and PANC1 (MUC4−) cell pellets while HPAFII and HPAC pellets that are known to express large amounts of MUC4 were strongly and diffusely positive. Some non-specific, acellular staining is seen within Mia PACA2 (MUC4−) cells.
Western Blot of Mouse Anti-MUC4 Antibody. Lane 1: Spectra Multicolor Ladder. Lane 2: HPAF-II (MUC4+). Lane 3: HPAC (MUC4+). Lane 4: Capan-2 (MUC4+). Lane 5: MiaPaCa2 (MUC4-). Lane 6: Panc-1 (MUC4-). Primary Antibody: Anti-MUC4 at 1nM in 2% BSA at RT for 2 hrs. Secondary Antibody: Goat Anti-Mouse IgG HRP (610-103-121) at 1:70,000 in 5% BSA/PBS/0.1 Tween 20 for 1 hr at RT. Expect: ~550-930 kDa. Observed: ~200-300 kDa. Highly glycosylated protein.
ELISA results from BSA constructs (0.3μg/mL) coated on plates and screened using the following antisera: anti-5TFag-TRMUC4 serum (1:300), affinity pure anti-5TFag-TRMUC4 polyclonal antibody (15μg/mL) and unimmunized rabbit serum (1:300). Primary antisera were added to well plates and serially diluted by half Log10 dilutions. Assay performed using Blocking buffer (3 % BSA), alkaline phosphatase conjugated secondary antibodies, and PNPP (10 mg) in pH 9.8 DEA buffer.
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Immunohistochemistry of Mouse Anti-MUC4 Antibody. Tissue/Cell: A) hu Mia PACA2 [-]. B) hu Capan2 pancreatic ductal adenocarcinoma cell line [+]. C) hu HPAFII cell pellet [+]. D) Control mouse liver [-]. E) hu Control PANC1 cell pellet [-]. F) hu HPAC cell pellet [+]. Tissues: FFPE containing normal and malignant pancreatic tissues. Cell pellets: Appr. 10 million cells were collected from flasks without enzymatic treatment and centrifuged; thrombin and Fibrinogen was added. The resulting pellet of clotted cells was collected. Fixative: 10% neutral buffered formalin at RT. Antigen Retrieval: HIER citrate buffer for 20 min. Primary Antibody: Anti-MUC4 Antibody at 1:1000 in A, B, C, F. Isotype control reagent used in negative control D, E. Secondary Antibody: Anti-Mouse IgG. Counter Stain: Bond Polymer Refine Detection Kit. Analysis Results: No staining was observed for control liver and PANC1 (MUC4−) cell pellets while HPAFII and HPAC pellets that are known to express large amounts of MUC4 were strongly and diffusely positive. Some non-specific, acellular staining is seen within Mia PACA2 (MUC4−) cells.
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| 別品名 |
Mouse Anti-MUC4 Antibody, Mouse Anti-Mucin 4 Antibody, MUC4, Mucin-4, Ascites sialoglycoprotein, ASGP, Pancreatic adenocarcinoma mucin, Testis mucin, Tracheobronchial mucin, Mucin-4 alpha chain, Ascites sialoglycoprotein 1, ASGP-1, Mucin-4 beta chain, Ascites sialoglycoprotein 2, ASGP-2, MUC
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| 交差種 |
Human
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
Immunohistochemistry
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| 免疫動物 |
Mouse
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| クローン |
3C7.D8.E11.F5
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| 標識物 |
Unlabeled
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| GENE ID |
4585
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| Accession No.(Gene/Protein) |
NP_004523.3, Q99102
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| Gene Symbol |
MUC4
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| 参考文献 |
[Pub Med ID]30952968
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| メーカー |
品番 |
包装 |
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RKL
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200-301-GY2
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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