Anti HIV-1 Reverse Transcriptase  

Fig.1 Detection of HIV-1 reverse transcriptase in the extract of HIV-1 infected cells by Western blotting using anti-HIV transcriptase antibody.
Lnae 1: Extract of MT4 cells
Lane 2: Extract of MT4 cells infected with HIV-1 (LAI strain)
The antiserum was diluted 2,500 fold before use.

Fig.2. Western blotting of functional recombinant full-length HIV-1 reverse transcriptase (#05-001) by using anti-HIV-1 Reverse Transriptase antibody.
1; 40 ng / lane
2; 20 ng / lane
3; 4 ng / lane
4; 2 ng /lane
Anti-HIV-1 RT antibody was used at 1/2,000 dilution. As second antibody, goat anti-rabbit IgG antibody conjugated with HRP was used at 1/5,000 dilution. ECL system was used.