X-Gal (β-Galactosidase Substrate)  

X-Gal (Beta-Galactosidase Substrate)

Neural ensembles in IL reactive to S+ or S- following the activity-targeted disruption by Daun02. Each of the four experimental groups, prepared and used for the Daun02 disruption and Post-disruption tests I, was further randomly divided into three groups defined by the type of final Fos induction condition (S+, S- or No S+/S-). The rats were exposed to S+, S- or No S+/S- (control), then deeply anesthetized and euthanized. Brains were collected, sectioned (40 μm), processed for Fos immunohistochemistry. Fos-positive nuclei from sampling areas around the IL microinjection sites were quantified double-blindly. X-gal (p/n XGAL-0100). The average numbers of Fos-positive nuclei per mm2？were calculated for each rat and used for statistical analyses. Yellow arrows represent typical Fos-positive nuclei. Group means of these average numbers (+SEM) are depicted. N？=？7？9, each. *p<0.05？0.01 (vs. No active lever/light-cue). Figure 3. PMID: 27938664.

Omission cue-induced suppression (OCIS) procedures for localization and phenotypic characterization of omission cue-reactive neurons in IL. All data are mean and SEM. Gray open circles on bar charts depict individual data-points.?a,?b,?c?Timeline and schedule.?d?Target sites.?e,?f?Representative sections.?g?Effects of cocaine S-, alcohol S-, and well-habituated odor on neural activation in IL as indicated by Fos immunohistochemistry.?n?=?10,9,8,9,6,6. Two-way between-subjects ANOVA: Training (F(2,42)?=?4.81,?P?