TMB Membrane Peroxidase Substrate  

TMB Membrane Peroxidase Substrate - Ready-To-Use

Rockland TMB membrane substrate, chemiluminescent substrate and secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with Rockland HRP conjugated Goat anti Rabbit IgG (611-103-122) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate (TMBM-100) for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager,

Immunological Features in the H1N1 Influenza-Infected SOTR Cohort(A and B) Pre-existing antibody titers in SOTRs with H1N1pdm09 influenza virus infection (n？= 40) compared with non-infected matched SOTRs controls (n？= 9). The antibody level for each patient, GMT, and 95% CI are shown (A) for the indicated ELISA assay on the left y axis (full-length HA, stalk HA, and NA recombinant proteins as antigen) and HA-inhibiting (HAI) antibody titers on the right y axis. The HAI assay was performed using the whole H1N1pdm09 reference strain virus. (B) shows antibody-dependent cellular cytotoxicity (ADCC), measured by a reporter assay for full-length HA, stalk HA, and NA.(C？F) Pre-existing antibody responses in SOTRs with LRSs at baseline. The antibody level for each patient and time point, GMT, and 95% CI are shown for (C) full-length HA ELISA, (D) stalk HA ELISA, (E) HAI, and (F) NA ELISA (ns, not significant; ？？？p？= 0.01; ？？p？= 0.006; ？p？= 0.03).Experiments were performed in triplicates. 3,3′,5,5′-Tetramethylbenzidine (p/n TMBM-100). Figure？1. PMID: 33294855.