Protein A  
SEPHAROSE (商標名)

SEPHAROSE？ Protein A

Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain. Protein A and G each show some preferences to certain types of antibody subclasses, and proper reagent selection may be required, Protein A/G offers the widest range of antibody subclass binding. Another immunoglobulin binding protein is Protein L which binds to the kappa light chain of the F(ab’)2 region.

Abelson kinase induces phosphorylation of TACC3 Abelson kinase induces phosphorylation of TACC3. (a) Western blot performed with phospho-tyrosine specific antibody showing Abelson-induced tyrosine phosphorylation of full-length TACC3 (lane 4). Phosphorylation signal is not present when TACC3 is expressed alone (lane 2) or with another tyrosine kinase Fyn (lane 6).？ Total protein was incubated with α-GFP and 20μL of a 50% slurry of sepharose Protein A (p/n PA50-00-0002) and Protein G resin prewashed with lysis buffer. FIGURE 1. PMID: 32543081.

BRD4 directly interacts with eRNAs through its tandem bromodomains. d,？f,？In vitro？pull-down of？MMP9 and CCL2？eRNAs with the indicated FLAG-tagged BRD4 proteins and？g, the His-tagged tandem BDs of BRD2, BRD3, BRD4, and BRDT and the single BDs of BRG1 and BRD7 as revealed by SYBR Gold staining and SDS-PAGE/Coomassie staining analyses of the purified proteins.？Cleared lysates were incubated with indicated antibodies followed by an additional incubation with Protein A Sepharose (p/n PA50-00-0002). Fig 3. PMID: 30076409.

Mutp53 and NFκB interact and impact each other’s binding at enhancers.？a？Purified wild-type and mutp53 proteins bind directly to purified NFκB/p65 as revealed by immunoblot analysis with an antibody that recognizes p65. Input samples for the p53 proteins were also analyzed by immunoblot analysis with an antibody that recognizes both wild-type and mutp53. Three independent interaction assays were performed. Fig. 3. PMID: 28963538.