Blocking Buffer for Fluorescent Western Blotting  

Blocking Buffer for Fluorescent Western Blotting

Selected sections of the PEPperCHIPR Peptide Microarrays after assay with different blocking reagents. The microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Rockland Blocking Buffer [p/n MB-070] (D), respectively. A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat Anti-Human IgG (H+L) DyLightTM 680 Antibody [p/n 609-144-123]. Red spots = sample responses and polio control peptides, green spots = HA control peptides. The underlying binding motifs of the respective sections are indicated on the left.

Comparison of the performance of different blocking reagents in epitope mappings with PEPperCHIPR Peptide Microarrays.The PEPperCHIPR Peptide Microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Rockland Blocking Buffer [p/n MB-070] (D). A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat anti-Human IgG (H+L) DyLight？ 680 Antibody [p/n 609-144-123] and a control anti-HA (12CA5)-DyLight？ 800 Antibody. Red spots = sample IgG response and frame of polio control peptides, green spots = frame of HA control peptides.