| 別品名 |
IRDye Blocking Buffer, Fluorescent Blocking Buffer, Blocking Solution, Blocking Buffer Western Blot, IRDye Western Blot Blocking Buffer, Alexa Dye Blocking Buffer
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| 適用 |
Western Blot
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| 形状 |
滅菌済み液状品
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| 使用目的 |
このブロッキングバッファーは、蛍光標識抗体を用いたウェスタンブロッティング用に特別にデザインされています。ご使用にあたってはニトロセルロースメンブレンを推奨しています。
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| 参考文献 |
[Pub Med ID]33903110, 25944688, 26668058, 29028839, 30012581, 30674877, 30723476, 31812776, 31900453, 31992583, 32788237, 32858147, 32923161, 33020308, 33148534, 33394033, 33661725, 33669483, 19638217, 32463974+他100件以上
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※サムネイル画像をクリックすると拡大画像が表示されます。
Comparison of the performance of different blocking reagents in epitope mappings with PEPperCHIPR Peptide Microarrays.The PEPperCHIPR Peptide Microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Rockland Blocking Buffer [p/n MB 070] (D). A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat anti Human IgG (H+L) DyLightTM 680 Antibody [p/n 609 144 123] and a control anti HA (12CA5) DyLightTM 800 Antibody. Red spots = sample IgG response and frame of polio control peptides, green spots = frame of HA control peptides.
Selected sections of the PEPperCHIPR Peptide Microarrays after assay with different blocking reagents. The microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Rockland Blocking Buffer [p/n MB-070] (D), respectively. A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat Anti-Human IgG (H+L) DyLightTM 680 Antibody [p/n 609-144-123]. Red spots = sample responses and polio control peptides, green spots = HA control peptides. The underlying binding motifs of the respective sections are indicated on the left.
This blocking buffer is specifically designed for western blotting using fluorochrome conjugated antibodies and can be used for membrane blocking and to dilute both primary and secondary antibodies. See www.rockland.com for specific protocols. This buffer was prepared using ultra-pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
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Comparison of the performance of different blocking reagents in epitope mappings with PEPperCHIPR Peptide Microarrays.The PEPperCHIPR Peptide Microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Rockland Blocking Buffer [p/n MB 070] (D). A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat anti Human IgG (H+L) DyLightTM 680 Antibody [p/n 609 144 123] and a control anti HA (12CA5) DyLightTM 800 Antibody. Red spots = sample IgG response and frame of polio control peptides, green spots = frame of HA control peptides.
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| メーカー |
品番 |
包装 |
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RKL
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MB-070
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500 ML
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
劇
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| 保存温度 |
４℃禁凍結
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