| 別品名 |
Anti-NFKB p50, Anti-NFKB p65, Rabbit Anti-NFKB cRel, Anti-IKBalpha, Control peptides, antibody sample kits
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| 標識物 |
Unlabeled
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| 適用 |
Western Blot
Enzyme Linked Immunosorbent Assay
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| 形状 |
滅菌済み液状品
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| 使用目的 |
ヒトNFκBとIκBの解析用に適した抗体キットです。これらはウェスタンブロッティングやゲルスーパーシフトアッセイ、免疫沈降、免疫染色、ELISAなどにお使いいただけます。
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| 構成内容 |
・Anti-NFκB p50 (NFKB1)
・Anti-NFκB p65 (RelA)
・Anti-c-Rel and Anti-IκBα 抗体各100μL
・コントロールPeptides
・Peroxidase Conjugated Affinity Purified Anti-Rabbit IgG [H&L] (Goat)
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western blot of HeLa cell extract. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed with anti NFkB antibody 100 4165. All incubations except color development were performed using TBS supplemented with 0.1% Tween 20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat a Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.
This product is assembled as a kit. See attached protocol or CofA for further details.
Western blot of HeLa cell extract using p/n 100-4166 Anti-NFKB cRel (RABBIT) Antibody (100 uL) . All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.
Western blot of HeLa cell extract using Anti-NFKB p50 (NFKB1) Antibody 100-4164.？ All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.？ The membrane was blocked in 5% dry milk for 2 h.？ After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.？ Washes with buffer were performed 4 times for 5' each.？ The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h.？？ Washes with TBS preceded color development.
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Western blot of HeLa cell extract. A predominant band ~65 kDa (arrowhead) corresponding to NFkb p65 Rel A is observed with anti NFkB antibody 100 4165. All incubations except color development were performed using TBS supplemented with 0.1% Tween 20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:5,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat a Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.
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| メーカー |
品番 |
包装 |
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RKL
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K711
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1 KIT
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 保存温度 |
-２０℃
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