Serum (Sterile)  

Donkey Serum is used as a supplement to cell culture media. Donkey Serum provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth and health. Be certain to maintain Good Cell Culture Practice, and maintain sterility of cultures that require media supplementation.

Loss of Stx5S compromises ER？Golgi trafficking. (a)？Immunofluorescence microscopy of GALNT2 (green in merge) in primary human dermal fibroblasts of healthy donors (green, Ctrl) or Stx5M55V patients (orange, Stx5M55V) in the absence or presence of brefeldin A (BFA) for 6？min and washout for the indicated times. Representative confocal micrographs are shown. Following quenching with 50？mM NH4Cl in PBS, cells were permeabilized and blocked in 2% normal donkey serum (p/n D120-00-0050) and 0.1% saponin (permeabilization buffer) for 30？min at RT. Scale bars, 10？μm. DAPI in blue. Fig. 5. PMID: 34711829.