Anti IgG (H&L)

DyLightTM dyes can be used for two color Western Blot detection with low background and high signal.？ Anti tubulin was detected using a DyLightTM 680 conjugate.？ Anti TNFa was detected using a DyLightTM 800 conjugate. The image was captured using the OdysseyR Infrared Imaging System developed by LI COR.

Properties of DyLightTM Conjugates.

Dot Blot Results of Goat Anti-Rat IgG Antibody DyLight？800 Conjugated (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rb, Sh Serum Proteins). RAT IgG whole molecule at 100ng, 33.33ng, 11.11ng, 3.70ng, 1.23ng. Antibody: Goat Anti-Rat IgG Antibody DyLight？800 Conjugated MX10 at 1μg/mL for 60mins at RT. Buffer: Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 30 mins at RT. Exposure: 37 sec.

Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-101-120). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-101-120) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.

DyLight？ dyes can be used for two-color Western Blot detection with low background and high signal.？ Anti-tubulin was detected using a DyLight？ 680 conjugate.？ Anti-TNFa was detected using a DyLight？ 800 conjugate. The image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.