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Penetration and distribution of neural crest-derived cells (NCDCs) in the?P0-Cre/EGFP?mouse telencephalon. (G?H′′′) A coronal section at the telencephalic level of the E9.5 embryo is stained with anti-laminin, Flk1, GFP antibodies and counter-stained 4′, 6-diamidino-2-phenylindole (DAPI). High-magnification images within the squares in G?G′′′ are shown in H?H′′′. Through the laminin+?basement membrane (H), an endothelial cell (white dashed line in H′) invades the telencephalon together with an EGFP+?cell (yellow line in H′′). Scale bars: (for G?G′′′), 50?μm; H′′′ (for H?H′′′), 25?μm. Figure 2. PMID: 23157329.
Existence of non-pericyte enhanced green fluorescent protein (EGFP+) cells in the telencephalon of E11.5?P0-Cre/EGFP?mice. (A-B) A coronal section at the telencephalic level is stained with anti-GFP, PDGFRβ, and NG2 antibodies. Most of EGFP+?cells express both PDGFRβ and NG2 (yellow arrowheads). (B) High-magnification of the dashed square in A′′. PDGFRβ is also expressed in hematopoietic cells (open arrowheads in A′?B). Fig 5. PMID: 23157329.
The usability of PDGFRβ and NG2 as pericyte markers in the telencephalon. (A?E′′) Immunostaining with anti-PDGFRβ, Flk1, and laminin antibodies on coronal section at the telencephalic level of E11.5?P0-Cre?mouse embryo. (A?D) A PDGFRβ+?pericyte (yellow arrowheads) wraps around Flk1+?endothelial tube (white arrowheads), and these cell types are surrounded by laminin+?basement membrane. This pattern is also clearly observed in orthogonal view of the image shown in D (E?E′′). Fig 3. PMID: 23157329.
DyLight? dyes can be used for two-color Western Blot detection with low background and high signal.? Anti-tubulin was detected using a DyLight? 549 conjugate.? Anti-TNFa was detected using a DyLight? 649 conjugate. The image was captured using the Typhoon? 9410 Imaging System.
Properties of DyLight? Conjugates.
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Penetration and distribution of neural crest-derived cells (NCDCs) in the?P0-Cre/EGFP?mouse telencephalon. (G?H′′′) A coronal section at the telencephalic level of the E9.5 embryo is stained with anti-laminin, Flk1, GFP antibodies and counter-stained 4′, 6-diamidino-2-phenylindole (DAPI). High-magnification images within the squares in G?G′′′ are shown in H?H′′′. Through the laminin+?basement membrane (H), an endothelial cell (white dashed line in H′) invades the telencephalon together with an EGFP+?cell (yellow line in H′′). Scale bars: (for G?G′′′), 50?μm; H′′′ (for H?H′′′), 25?μm. Figure 2. PMID: 23157329.
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| 別品名 |
Goat Anti-Rat IgG DyLight 649TM Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 649TM Conjugation
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| 交差種 |
Rat
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| 非交差(吸収処理)種 |
Human Mouse Bovine Rabbit Chicken Sheep Goat Guinea Pig Hamster Equine
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| 免疫動物 |
Goat
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| 標識物 |
DyLightTM 649
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| 精製度 |
Affinity Purified
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| 参考文献 |
[Pub Med ID]23157329
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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612-143-120
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
毒
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| 保存温度 |
4℃
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