Anti IgG F(c)

Dot Blot of Goat anti Rat IgG Fc Antibody Fluorescein Conjugated. Antigen: Rat IgG. Load: Lane 1 100 ng Lane 2 33.3 ng Lane 3 11.1 ng Lane 4 3.70 ng Lane 5 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti Rat IgG Fc Antibody Fluorescein Conjugated at 1:1,000 for 60 min at RT. Block: MB 070 for 1 HR at RT.

Western Blot of Anti-Rat IgG F(c) (GOAT) Antibody (p/n 612-1103). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG F(c) (GOAT) Antibody (p/n 612-1103) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.