Anti IgG (H&L)  

Western Blot of Donkey anti-Rabbit IgG (H&L) Antibody DyLight？ 488 Conjugated Pre-Adsorbed. Lane 1: Rabbit IgG. Load: 50 ng per lane. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight？ 488 Conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa.

Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-701-127). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), F(c) and Serum, 25 ng of IgM. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-701-127) 1:7,500 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

Properties of DyLight？ Fluorescent Dyes.

DyLight？ dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image.？ DyLight？ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods.？ This image shows anti-histone detection using a DyLight？ 488 conjugate (green).？ Anti-Tubulin was detected using a DyLight？ 549 conjugate (red). ？Nuclei were counter-stained using DAPI (blue).？ The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).