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Western Blot of Anti Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611 701 127). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011 0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011 0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010 0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011 0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), F(c) and Serum, 25 ng of IgM. Block: MB 070 for 30 min at RT. Primary Antibody: Anti Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611 701 127) 1:7,500 for 60 min at RT. Secondary antibody: Anti Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616 1302) 1:40,000 in MB 070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Western blot of Fluorescein conjugated Donkey Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
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Western Blot of Anti Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611 701 127). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011 0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011 0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010 0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011 0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), F(c) and Serum, 25 ng of IgM. Block: MB 070 for 30 min at RT. Primary Antibody: Anti Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611 701 127) 1:7,500 for 60 min at RT. Secondary antibody: Anti Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616 1302) 1:40,000 in MB 070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
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メーカー |
品番 |
包装 |
RKL
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611-702-127
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1 MG
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※表示価格について
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