種由来 |
Rabbit
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Guinea Pig
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非交差(吸収処理)種 |
Human Mouse Goat
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]34901015
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
ELISA Results of Purified Guinea Pig Anti Rabbit IgG mx3 Antibody tested against purified Rabbit IgG (Mx Human IgG, Goat IgG, Mouse IgG). Each well was coated in duplicate with 10 ug of Rabbit IgG (p/n 011 0102) [Red Line], Human IgG (p/n 009 0102) [Green Line], Goat IgG (p/n 005 0102) [Blue Line], Mouse IgG (p/n 010 0102) [Purple Line]. The working dilution is 1:17,000. The starting dilution of antibody was 5μg/ml and the X axis represents the Log10 of a 3 fold dilution. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB 066), Goat Anti Guinea Pig mx10 HRP conjugated (p/n 606 103 129) and TMB substrate (p/n TMBE 1000).
Western Blot of Anti-Rabbit IgG (H&L) (GUINEA PIG) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-201-122). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GUINEA PIG) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-201-122) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Pig IgG (GOAT) Peroxidase Conjugated Antibody (p/n 606-103-129) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.
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ELISA Results of Purified Guinea Pig Anti Rabbit IgG mx3 Antibody tested against purified Rabbit IgG (Mx Human IgG, Goat IgG, Mouse IgG). Each well was coated in duplicate with 10 ug of Rabbit IgG (p/n 011 0102) [Red Line], Human IgG (p/n 009 0102) [Green Line], Goat IgG (p/n 005 0102) [Blue Line], Mouse IgG (p/n 010 0102) [Purple Line]. The working dilution is 1:17,000. The starting dilution of antibody was 5μg/ml and the X axis represents the Log10 of a 3 fold dilution. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB 066), Goat Anti Guinea Pig mx10 HRP conjugated (p/n 606 103 129) and TMB substrate (p/n TMBE 1000).
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メーカー |
品番 |
包装 |
RKL
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611-201-122
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1 MG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃
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