別品名 |
Rabbit Antibody alkaline phosphatase conjugated, Gt-a-Rabbit alkaline phosphatase conjugated IgG, Rabbit alkaline phosphatase conjugated Antibody in Goat, Rabbit Secondary alkaline phosphatase conjugated Antibody, ALP, Alk Phos.
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種由来 |
Rabbit
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標識物 |
Alkaline Phosphatase
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Dot Blot
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免疫動物 |
Goat
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交差種 |
Rabbit
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形状 |
滅菌済み液状品
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参考文献 |
[Pub Med ID]11587551, 20219539, 8781330, 32038740
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
ELISA results of Goat Anti Rabbit IgG Antibody Alkaline Phosphatase Conjugated tested against purified Rabbit IgG protein. Each well was coated in duplicate with 10 ug of Rabbit IgG (p/n 011 0102). The starting dilution of antibody was 5μg/ml and the X axis represents the Log10 of a 3 fold dilution. The titer is 1:45,700. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE 1000.
Dot Blot of Rabbit IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Rabbit IgG. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng. Primary antibody: none. Secondary antibody: Rabbit IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
Western Blot of Goat anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated. Lane 1: Rabbit IgG. Lane 2: None. Load: 100 ng per lane. Primary Antibody: None. Secondary antibody: Alkaline Phosphatase goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 kDa for Rabbit IgG. Other Band(s): None.
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ELISA results of Goat Anti Rabbit IgG Antibody Alkaline Phosphatase Conjugated tested against purified Rabbit IgG protein. Each well was coated in duplicate with 10 ug of Rabbit IgG (p/n 011 0102). The starting dilution of antibody was 5μg/ml and the X axis represents the Log10 of a 3 fold dilution. The titer is 1:45,700. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE 1000.
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メーカー |
品番 |
包装 |
RKL
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611-1502
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1 MG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃禁凍結
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