Anti IgG (H&L)

Rabbit IgG (H&L) (Goat) Antibody DyLightTM 800 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)

ELISA of DyLightTM 800 Conjugated Goat Anti-Rabbit Secondary Antibody. Antigen: HCT-116 cell line. Coating amount: Confluent in the 96 well plate. Primary antibody: AKT or GAPDH antibody at 2 ug/mL. Dilution series: Primary and Secondary Antibodies 2-fold. Mid-point concentration: N/A. Secondary antibody: DyLightTM 680 donkey secondary antibody and DyLightTM 800 goat secondary antibody starting at 1:1,000. Substrate: None.

DyLight？ dyes can be used for two-color Western Blot detection with low background and high signal.？ Anti-tubulin was detected using a DyLight？ 680 conjugate.？ Anti-TNFa was detected using a DyLight？ 800 conjugate. The image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.

Properties of DyLightTM Conjugates.

Western Blot of Unconjugated Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.