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C. Representative western blots, original blots are shown in (supplementary Fig S8-9). And densitometric quantification of relative protein levels from western blots. Data are depicted as mean ± SD, n = 3, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by one-way ANOVA. Intracellular transport, activation, mitochondrial transport, β-oxidation, carnitine shuttle, and auxiliary proteins. The primary antibodies used as follows: VLCAD 1:1000, MCAD 1:1000, LCAD 1:1000, TFPa 1:500, TFPb 1:3000, CPT1α 1:1000, and GAPDH 1:30,000 dilutions overnight at 4 °C. The membranes were then incubated with fluorescent conjugated secondary antibodies for 1 h; DyLight 800 conjugated goat Anti-Rabbit IgG (611-145-002), DyLight 680 conjugated goat Anti-Rabbit IgG (611-144-003), DyLight 800 conjugated goat Anti-Mouse IgG (610-145-002), and DyLight 680 conjugated donkey Anti-Mouse IgG (610-744-124). Fig 1. PMID: 33725513.
Assessment of mitochondrial fusion and fission. B. Representative western blots (original blots are shown in supplementary Fig. S10) and quantification of MFN1/2 and DRP1. No significant changes in the relative levels of proteins that facilitate mitochondrial fusion (MFN1/2) and fission (DRP1) between non-disease (control) and mutant primary fibroblasts. Data are depicted as mean ± SD, n = 3. The primary antibodies used as follows: MFN1 1:400, MFN2 ( 1:400, DRP1 1:100 and GAPDH 1:30,000 dilutions overnight at 4 °C. The membranes were then incubated with fluorescent conjugated secondary antibodies for 1 h; DyLight 800 conjugated goat Anti-Rabbit IgG (611-145-002), Antibody DyLight 680 conjugated Anti-Rabbit IgG made in goat (611-144-003), DyLight 800 conjugated goat Anti-Mouse IgG (610-145-002), and DyLight 680 conjugated donkey Anti-Mouse IgG (610-744-124). Fig 3. PMID: 33725513.
ELISA of DyLight？ 680 Conjugated Donkey Anti-Mouse Secondary Antibody. Antigen: HCT-116 cell line. Coating amount: Confluent in the 96 well plate. Primary antibody: AKT or GAPDH antibody at 2 μg/mL. Dilution series: Primary and Secondary Antibodies 2-fold. Mid-point concentration: N/A. Secondary antibody: DyLight？ 680 donkey secondary antibody and DyLight？ 800 goat secondary antibody starting at 1:1,000. Substrate: None.
Priming Results in Increased Frequency of Activation and Enhanced？GBP5？Expression upon Reinduction.(F) EGFP::GBP5 cells were subjected to the IFNγ treatment regimen outlined in？Figure？1B, processed for fluorescence western blotting, and probed for GBP5 and EGFP expression. α-TUB, loading control. Tubulin-normalized fluorescence intensities are plotted. Fig 3. PMID: 33108759.
Depletion of human SPT6 leads to the loss of CENP-A maintenance. HeLa cells expressing SNAP-tagged CENP-A were treated with TMR-star to detect previously incorporated CENP-A and siRNA-treated to deplete proteins indicated in (b,？c). Cells were then synchronized in S phase by a thymidine block and released. Cells were allowed transit through G1 phase and were collected at the next G1/S boundary by re-addition of thymine.？b？Cells were treated with indicated siRNAs for 48？h and extracts were processed for immunoblotting and probed with indicated antibodies. CC CENP-C, M Marker.？N？=？3 independent experiments. Fig 6. PMID: 32522980.
DyLight？ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight？ 680 conjugate. Anti-TNFa was detected using a DyLight？ 800 conjugate. The image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.
Properties of DyLight？ Conjugates.
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C. Representative western blots, original blots are shown in (supplementary Fig S8-9). And densitometric quantification of relative protein levels from western blots. Data are depicted as mean ± SD, n = 3, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by one-way ANOVA. Intracellular transport, activation, mitochondrial transport, β-oxidation, carnitine shuttle, and auxiliary proteins. The primary antibodies used as follows: VLCAD 1:1000, MCAD 1:1000, LCAD 1:1000, TFPa 1:500, TFPb 1:3000, CPT1α 1:1000, and GAPDH 1:30,000 dilutions overnight at 4 °C. The membranes were then incubated with fluorescent conjugated secondary antibodies for 1 h; DyLight 800 conjugated goat Anti-Rabbit IgG (611-145-002), DyLight 680 conjugated goat Anti-Rabbit IgG (611-144-003), DyLight 800 conjugated goat Anti-Mouse IgG (610-145-002), and DyLight 680 conjugated donkey Anti-Mouse IgG (610-744-124). Fig 1. PMID: 33725513.
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| 別品名 |
Donkey anti-Mouse IgG DyLight 680TM Conjugated Antibody, Donkey anti Mouse IgG Antibody DyLight 680TM Conjugation
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| 交差種 |
Mouse
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| 非交差(吸収処理)種 |
Human
Rat
Bovine
Rabbit
Chicken
Sheep
Goat
Guinea Pig
Hamster
Equine
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| 適用 |
Enzyme Linked Immunosorbent Assay
Dot Blot
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| 免疫動物 |
Donkey
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| 標識物 |
DyLightTM 680
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| 精製度 |
Affinity Purified
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| 参考文献 |
[Pub Med ID]33108759
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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610-744-124
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
毒
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| 保存温度 |
４℃
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