Anti IgG (H&L)

Rockland Rabbit anti Transferrin (109 4134 lot 3033, green), Goat anti Alpha 1 Anti Trypsin (100 101 147 lot 5842), and Mouse a GST (200 301 200 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS PAGE, transferred to 0.2 um PVDF using the BioRad Trans Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB 070 over night at 4C). Detection shown was using DyLight549 Donkey anti Rabbit IgG (611 742 127 lot 21100, shown as green) ？ DyLight 488 Donkey anti Mouse IgG (610 741 124 lot 21095, shown as blue), ？and DyLight 649 Donkey anti Goat IgG (605 743 125 lot 20834, shown as red) at 1:10000 (in MB 070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.

Properties of DyLightTM Fluorescent Dyes.

DyLight？ dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image.？ DyLight？ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods.？ This image shows anti-Histone detection using a DyLight？ 488 conjugate (green).？ Anti-Tubulin was detected using a DyLight？ 549 conjugate (red). ？Nuclei were counter-stained using DAPI (blue).？ The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).

Western Blot of Fluorescein conjugated Donkey Anti-Mouse IgG Pre-Adsorbed secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.

Rockland Rabbit anti-Transferrin (109-4134 lot 3033, green), Goat-anti-Alpha-1-Anti-Trypsin (100-101-147 lot 5842), and Mouse-a-GST (200-301-200 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (611-742-127 lot 21100, shown as green) ？ DyLight 488 Donkey anti-Mouse IgG (610-741-124 lot 21095, shown as blue), ？and DyLight 649 Donkey anti-Goat IgG (605-743-125 lot 20834, shown as red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.