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Properties of DyLightTM Fluorescent Dyes.
Properties of DyLightTM Fluorescent Dyes.
DyLightTM dyes can be used for two-color Western Blot detection with low background and high signal.? Anti-tubulin was detected using a DyLightTM 549 conjugate.? Anti-TNFa was detected using a DyLightTM 649 conjugate. The image was captured using the TyphoonTM 9410 Imaging System.
Western Blot of Goat anti-Mouse IgG (H&L) Antibody DyLightTM 649 Conjugated Pre-Adsorbed Lane 1: Mouse IgG Load: 50 ng per lane Secondary antibody: Mouse IgG (H&L) Antibody DyLightTM 649 Conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed Size: 28 and 55 kDa/28 and 55 kDa
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