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Rockland Dylight 549 Goat Anti Mouse IgG antibody Immunofluorescence Cell Type: A431 cells Fixation: 4% paraformaldehyde 10 min Permeabilization: 0.5% Triton X 30 min Primary Ab: 200 301 880 lot 28977 1:250 72 hours 4C Secondary Ab: 610 142 121 lot 21290 1:1000 Overnight 4C
Properties of DyLightTM Conjugates.
DyLight？ dyes can be used for two-color western blot detection with low background and high signal.？ Anti-tubulin was detected using a DyLight？ 549 conjugate.？ Anti-TNFa was detected using a DyLight？ 649 conjugate. The image was captured using the Typhoon？ 9410 Imaging System.
Western Blot of DyLightTM 549 conjugated Goat anti-Mouse IgG Pre-adsorbed secondary antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: DyLightTM 549 goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): none.
Rockland Dylight 549 Goat Anti Mouse IgG antibody-Immunofluorescence Cell Type: A431 cells Fixation: 4% paraformaldehyde 10 min Permeabilization: 0.5% Triton X 30 min Primary Ab: 200-301-880 lot 28977 1:250 72 hours 4°C Secondary Ab: 610-142-121 lot 21290 1:1000 Overnight 4°C
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