Anti IgG (H&L)

ELISA Results of Goat Anti Mouse IgG Antibody tested against purified Mouse IgG protein. Each well was coated in duplicate with 1.0 ug of Mouse IgG (p/n 010 0102). The starting dilution of antibody was 5μg/ml and the X axis represents the Log10 of a 3 fold dilution. The titer is 1:35,800. This titration is a 4 parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Donkey Anti Goat IgG Peroxidase Conjugate at 1:40,000 and TMB substrate p/n TMBE 1000.

Western Blot of Goat Anti-Mouse IgG (H&L) Antibody. Lane M: Molecular Ladder. Lane 1: Mouse IgG whole molecule (p/n 010-0102). Lane 2: Mouse IgG F(c) Fragment (p/n 010-0103). Lane 3: Mouse IgG F(ab) Fragment (010-0105). Lane 4: Mouse IgM Kappa (p/n 010-001-339). Lane 5: Mouse Serum (p/n D308). Load: 50ng per lane. Block: (p/n MB-070) for 30 min at RT. Primary Antibody: Anti-Mouse IgG (H&L) Antibody 1:1000 for 60 min at RT. Secondary antibody: HRP Donkey Anti-Goat IgG (p/n 605-703-125) at 1:40,000 for 30 min at RT . Predicted/Observed Size: 28 and 55 kDa for Mouse IgG, F(c), F(ab), IgM Kappa, and Serum. Mouse F(c) migrates slightly higher.

Western Blot of Goat anti-Mouse IgG (H&L) Antibody. Lane 1: Mouse IgG. Load: 100 ng per lane. Primary Antibody: Mouse IgG (H&L) Antibody 1:1000 overnight at 4C. Secondary antibody: DyLightTM 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed Size: 28 and 55 kDa/28 and 55 kDa for Mouse IgG. Other band(s): none.