Anti IgG (H&L)  

Selected sections of the PEPperCHIP^(R) Peptide Microarrays after assay with different blocking reagents. The microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Rockland Blocking Buffer [p/n MB-070] (D), respectively. A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat Anti-Human IgG (H+L) DyLight^TM 680 Antibody [p/n 609-144-123]. Red spots = sample responses and polio control peptides, green spots = HA control peptides. The underlying binding motifs of the respective sections are indicated on the left.

Comparison of the performance of different blocking reagents in epitope mappings with PEPperCHIP^(R) Peptide Microarrays.The PEPperCHIP^(R) Peptide Microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Rockland Blocking Buffer [p/n MB-070] (D). A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat anti-Human IgG (H+L) DyLight^TM 680 Antibody [p/n 609-144-123] and a control anti-HA (12CA5)-DyLight^TM 800 Antibody. Red spots = sample IgG response and frame of polio control peptides, green spots = frame of HA control peptides.

DyLight^TM dyes can be used for two-color Western Blot detection with low background and high signal.？ Anti-tubulin was detected using a DyLight^TM 680 conjugate.？ Anti-TNFa was detected using a DyLight^TM 800 conjugate. The image was captured using the Odyssey^(R) Infrared Imaging System developed by LI-COR.

Properties of DyLight^TM Conjugates.