| 種由来 |
Human
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| 標識物 |
DyLightTM 680
|
| 精製度 |
Affinity Purified
|
| 適用 |
Dot Blot
|
| 免疫動物 |
Goat
|
| 非交差(吸収処理)種 |
Mouse
Rat
Bovine
Rabbit
Chicken
Sheep
Goat
Guinea Pig
Hamster
Equine
|
| 形状 |
凍結乾燥品
|
| 参考文献 |
[Pub Med ID]25944688, 29289663, 30023708, 32550563
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Comparison of the performance of different blocking reagents in epitope mappings with PEPperCHIPR Peptide Microarrays.The PEPperCHIPR Peptide Microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Rockland Blocking Buffer [p/n MB 070] (D). A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat anti Human IgG (H+L) DyLightTM 680 Antibody [p/n 609 144 123] and a control anti HA (12CA5) DyLightTM 800 Antibody. Red spots = sample IgG response and frame of polio control peptides, green spots = frame of HA control peptides.
Selected sections of the PEPperCHIPR Peptide Microarrays after assay with different blocking reagents. The microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Rockland Blocking Buffer [p/n MB-070] (D), respectively. A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat Anti-Human IgG (H+L) DyLightTM 680 Antibody [p/n 609-144-123]. Red spots = sample responses and polio control peptides, green spots = HA control peptides. The underlying binding motifs of the respective sections are indicated on the left.
DyLight？ dyes can be used for two-color Western Blot detection with low background and high signal.？ Anti-tubulin was detected using a DyLight？ 680 conjugate.？ Anti-TNFa was detected using a DyLight？ 800 conjugate. The image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.
Properties of DyLightTM Conjugates.
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Comparison of the performance of different blocking reagents in epitope mappings with PEPperCHIPR Peptide Microarrays.The PEPperCHIPR Peptide Microarrays were blocked for 30 minutes with either 2% skim milk powder (A), 1% HSA (B), 1% BSA (C) or 100% Rockland Blocking Buffer [p/n MB 070] (D). A human serum sample was assayed at dilution 1:200, followed by detection with secondary goat anti Human IgG (H+L) DyLightTM 680 Antibody [p/n 609 144 123] and a control anti HA (12CA5) DyLightTM 800 Antibody. Red spots = sample IgG response and frame of polio control peptides, green spots = frame of HA control peptides.
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| メーカー |
品番 |
包装 |
|
RKL
|
609-144-123
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100 UG
|
※表示価格について
| 当社在庫 |
なし
|
| 納期目安 |
約10日
|
| 法規制 |
毒
|
| 保存温度 |
４℃
|
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