Anti IgG (H&L)  

Western Blot analysis of IgG and IgM antibodies against？R. helvetica？whole cell antigen demonstrates the lipopolysaccaride (LPS) ladders and specific reactions against？R. helvetica？proteins in the 110？150-kDa region in serum for IgG for patients 1, 32 and 33 and for IgM for patients 3, 8, 10, 17, 20 and 22 in dilution 1:200. Lane P(h) demonstrates specific proteins and the LPS ladders reacting with a positive human serum and P(r) with a polyclonal rabbit antiserum. N(h) represent a negative human serum control. Secondary antibody Anti-human IgG DyLight？549 (p/n 609？142-123 ) and Anti-human IgM DyLight？ 549 (p/n 609？142-007). Figure 1. PMID: 34712390.

Western Blot analysis of IgG antibodies against？R.？helvetica？whole cell antigen. Lane A-P demonstrates the lipopolysaccaride ladders and specific reactions against？R.？helvetica？proteins in the 110-150-kDa region for serum 2 for patients (Lane) V16(A), V43(B), V46 (C)(Area V); S71(D), S72(E), S75(F), V6(G) (Area O); K7(H), K9(I), K14(J), K46(K) K56(L) (Area K); A13(M), A16(N), A23(O), A35(P) (Area A) in titres 1:200. Lane P(h) demonstrates specific proteins and the lipopolysaccharide (LPS) ladders reacting with a human antiserum from a patient diagnosed with rickettsial infection and N(h) a healthy negative blood donor. Mw = molecular weight marker. “Fig 2” is compiled of four figure panels representing the groups of lanes that originated from different gels/blots (Gel A-D). The short vertical lines of “Fig 2” divide the individual non-adjacent lanes in the gels. The original analyses are presented in？S1？S4？Figs with Gels A-D as Supporting Information. Fig 2. PMID: 27846275.

702 Peptides are printed in duplicates randomly distributed on the microarray. Control peptides (HA and FLAG controls) are located in a square surrounding the peptides of interest. As secondary antibody DyLight？ 549 conjugated goat anti-human IgG antibody and for the FLAG control peptide a mouse anti-FLAG-Cy3 antibody were used; microarrays were read using a Fujifilm Life Science FLA-5100 imaging system with a SHG 532nm (green) diode laser and an LPG filter. Fig e1. PMID: 26894206.

Properties of DyLight？ Conjugates.

DyLight？ dyes can be used for two-color western blot detection with low background and high signal. Anti-tubulin was detected using a DyLight？ 549 conjugate. Anti-TNFa was detected using a DyLight？ 649 conjugate. The image was captured using the Typhoon？ 9410 Imaging System.