Anti IgG (H&L)  

Immunofluorescence results using Donkey Anti-Goat IgG DyLight 800.Effect of mutation in YXXL domain of HRSV M protein.C) The mutation of the tyrosine at amino acid residue 197 to alanine and leucine at amino acid residue 200 to alanine causes a distinct phenotypic change in the localization of the HRSV Matrix protein. HEp2 cells were transfected with indicated pEGFP-C1 Opt. M constructs and were fixed with paraformaldehyde at 24 hours post transfection as described previously. Cells were imaged for Opt.M, adaptor protein and DAPI using CLSM protocols described previously. Negative controls include transfection of cells with empty plasmid vector (pEGFP-C1) without M insert (left side panel) and mock transfected cells with staining for adaptor protein only with goat anti Ap3u3A followed by rabbit anti goat Alexa Fluor 546 antibody (right side panel). The first 3 images on the left side panel shows staining for Opt.M and DAPI. The first 3 images on the right side panel shows merged image with staining for Opt.M, adaptor protein and DAPI. The results were reproducible in at least two independent assays.？Fig 4. PMID: 29028839.