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DyLightTM dyes can be used for two color Western Blot detection with low background and high signal.? Anti tubulin was detected using a DyLightTM 680 conjugate.? Anti TNFa was detected using a DyLightTM 800 conjugate. The image was captured using the OdysseyR Infrared Imaging System developed by LI COR.
Properties of DyLightTM Fluorescent Dyes
Western Blot of Donkey anti-Goat IgG Pre-Adsorbed DyLightTM 680 Conjugated Secondary Antibody. Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: DyLightTM 680 goat secondary antibody at 1:5,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): None.
DyLight? dyes can be used for two-color Western Blot detection with low background and high signal.? Anti-tubulin was detected using a DyLight? 680 conjugate.? Anti-TNFa was detected using a DyLight? 800 conjugate. The image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.
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