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Rockland Rabbit anti-Transferrin (109-4134 lot 3033, green), Goat-anti-Alpha-1-Anti-Trypsin (100-101-147 lot 5842), and Mouse-a-GST (200-301-200 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (611-742-127 lot 21100, shown as green) ？ DyLight 488 Donkey anti-Mouse IgG (610-741-124 lot 21095, shown as blue), ？and DyLight 649 Donkey anti-Goat IgG (605-743-125 lot 20834, shown as red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
B) Western blotting with anti-TRPV1 antibody of the proteins isolated from the urothelium (Uro), detrusor smooth muscle (DSM), and distal colon (Colon). The amount of protein loaded per lane was 40 μg for all tissues. Alpha tubulin was used as a loading control.？Fig. 1. PMID: 23146715.
DyLight？ dyes can be used for two-color Western Blot detection with low background and high signal.？ Anti-tubulin was detected using a DyLight？ 549 conjugate.？ Anti-TNFa was detected using a DyLight？ 649 conjugate. The image was captured using the Typhoon？ 9410 Imaging System.
Properties of DyLight？ Conjugates.
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Rockland Rabbit anti-Transferrin (109-4134 lot 3033, green), Goat-anti-Alpha-1-Anti-Trypsin (100-101-147 lot 5842), and Mouse-a-GST (200-301-200 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (611-742-127 lot 21100, shown as green) ？ DyLight 488 Donkey anti-Mouse IgG (610-741-124 lot 21095, shown as blue), ？and DyLight 649 Donkey anti-Goat IgG (605-743-125 lot 20834, shown as red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
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| 別品名 |
Donkey anti-Goat IgG Antibody DyLightTM 649 Conjugated Pre-Adsorbed, Donkey anti-Goat IgG DyLightTM 649 Conjugated Antibody
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| 交差種 |
Goat
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| 非交差(吸収処理)種 |
Mouse
Rat
Rabbit
Chicken
Guinea Pig
Hamster
Equine
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| 免疫動物 |
Donkey
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| 標識物 |
DyLightTM 649
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| 精製度 |
Affinity Purified
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| 参考文献 |
[Pub Med ID]23146715
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| [注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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| メーカー |
品番 |
包装 |
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RKL
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605-743-125
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100 UG
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※表示価格について
| 当社在庫 |
なし
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| 納期目安 |
約10日
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| 法規制 |
毒
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| 保存温度 |
４℃
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