別品名 |
Chicken Antibody, Rb-a-Chicken IgG, Chicken Antibody in Rabbit, Chicken Secondary Antibody
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種由来 |
Chicken
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標識物 |
Unlabeled
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精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay
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免疫動物 |
Rabbit
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交差種 |
Chicken
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形状 |
滅菌済み液状品
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[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Western Blot of Unconjugated Anti Chicken IgG (H&L) (RABBIT) Antibody (p/n 603 4102). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003 0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003 0103). Lane 3: Chicken IgG Fab Fragment (p/n 003 0105). All samples were reduced. Load: 50 ng per lane. Block: MB 070 for 30 min at RT. Primary Antibody: Anti Chicken IgG (H&L) (RABBIT) Antibody (p/n 603 4102) 1:3,000 for 60 min at RT. Secondary antibody: Anti Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611 103 122) 1:40,000 in MB 070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.
Western Blot of Rabbit anti-Chicken IgG Antibody. Lane 1: Chicken IgG (p/n 003-0102). Load: 100 ng per lane. Primary antibody: Chicken IgG antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLightTM 649 rabbit anti-Chicken secondary antibody (p/n 603-443-002) at 1:20,000 for 30 min at RT. Block: 5% BLOTTO (B501) for 30 min at RT. Predicted/Observed size: 72 and 28 kDa, 72 and 28 kDa for Chicken IgY.
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Western Blot of Unconjugated Anti Chicken IgG (H&L) (RABBIT) Antibody (p/n 603 4102). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003 0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003 0103). Lane 3: Chicken IgG Fab Fragment (p/n 003 0105). All samples were reduced. Load: 50 ng per lane. Block: MB 070 for 30 min at RT. Primary Antibody: Anti Chicken IgG (H&L) (RABBIT) Antibody (p/n 603 4102) 1:3,000 for 60 min at RT. Secondary antibody: Anti Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611 103 122) 1:40,000 in MB 070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.
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メーカー |
品番 |
包装 |
RKL
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603-4102
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10 MG
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※表示価格について
当社在庫 |
なし
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納期目安 |
約10日
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保存温度 |
4℃
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