Anti IgG (H&L)

DyLight^TM dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. DyLight^TM dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods. This image shows anti-histone detection using a DyLight^TM 488 conjugate (green). Anti-Tubulin was detected using a DyLight^TM 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc).

Western Blot of Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-101-126). Lane M: 3 μl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-101-126) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 72 kDa for Chicken IgG and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.

Properties of Dylight^TM Conjugates.