標識物 |
Biotin
|
精製度 |
Affinity Purified
|
適用 |
Western Blot Enzyme Linked Immunosorbent Assay
|
免疫動物 |
Rabbit
|
抗体クラス |
IgG
|
Tag情報 |
FLAG
|
形状 |
凍結乾燥品
|
[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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Rockland's antibody to detect FLAGTM conjugated proteins is shown to detect as little as 3 ng of amino terminal FLAGTM tagged recombinant protein by western blot. This antibody was used at a 1:1,000 dilution to detect 3 fold serial dilutions of amino terminal FLAGTM Bacterial Alkaline Phosphatase (BAP) fusion protein (Sigma P 7582) starting at 1.0 ug of protein as shown in lanes 1 6 respectively. A 4 20% gradient gel was used to separate the protein by SDS PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDyeR 800 conjugated Gt a Rabbit IgG (H&L) (code 611 132 122) for 30 min at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Affinity Purified Antibody to detect FLAGTM conjugated proteins detects both C terminal linked and N terminal linked FLAGTM tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2,500 to detect 1.0 ug of recombinant protein containing either the FLAGTM epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDyeR 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code 611-132-122) for 30 min at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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Rockland's antibody to detect FLAGTM conjugated proteins is shown to detect as little as 3 ng of amino terminal FLAGTM tagged recombinant protein by western blot. This antibody was used at a 1:1,000 dilution to detect 3 fold serial dilutions of amino terminal FLAGTM Bacterial Alkaline Phosphatase (BAP) fusion protein (Sigma P 7582) starting at 1.0 ug of protein as shown in lanes 1 6 respectively. A 4 20% gradient gel was used to separate the protein by SDS PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDyeR 800 conjugated Gt a Rabbit IgG (H&L) (code 611 132 122) for 30 min at room temperature. LICOR's OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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メーカー |
品番 |
包装 |
RKL
|
600-406-383
|
100 UG
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
法規制 |
毒
|
保存温度 |
4℃
|
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