Anti 6X His Tag  

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot.？ Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 μg/ml was used to detect 1.0 μg of recombinant protein containing the His epitope tag.？ A 4-20% gradient gel was used to resolve the protein by SDS-PAGE.？ The protein was transferred to nitrocellulose using standard methods.？ After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDyeR 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code 611-132-122) for 30 min at room temperature.？ LICOR's OdysseyR Infrared Imaging System was used to scan and process the image.？ Other detection systems will yield similar results.