抗原部位 |
a.a.410-419
|
種由来 |
Human
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標識物 |
Biotin
|
精製度 |
Affinity Purified
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適用 |
Western Blot Enzyme Linked Immunosorbent Assay Dot Blot
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免疫動物 |
Rabbit
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Tag情報 |
MYC
|
形状 |
凍結乾燥品
|
参考文献 |
[Pub Med ID]26966178
|
[注意事項] |
濃度はロットによって異なる可能性があります。メーカーDS及びCoAからご確認ください。
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※サムネイル画像をクリックすると拡大画像が表示されます。
Anti Myc epitope tag polyclonal antibody detects Myc tagged recombinant proteins by western blot.? Polyclonal rabbit anti Myc epitope tag at 0.5 1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the Myc epitope tag.? A 4 20% gradient gel was used to separate the protein by SDS PAGE.? The protein was transferred to nitrocellulose using standard methods.? After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDyeTM800 conjugated Gt a Rabbit IgG [H&L] (code 611 132 122) for 30 min at room temperature.?? LICOR's OdysseyR Infrared Imaging System was used to scan and process the image.? Other detection systems will yield similar results.
Western Blot of Biotin Conjugated Rabbit anti-Myc Epitope Tag antibody. Lane 1: 12-Tag Lysate. Lane 2: None. Load: N/A. Primary antibody: None. Secondary antibody: Biotin rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Myc Epitope Tag. Other band(s): Myc splice variants and isoforms.
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Anti Myc epitope tag polyclonal antibody detects Myc tagged recombinant proteins by western blot.? Polyclonal rabbit anti Myc epitope tag at 0.5 1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the Myc epitope tag.? A 4 20% gradient gel was used to separate the protein by SDS PAGE.? The protein was transferred to nitrocellulose using standard methods.? After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDyeTM800 conjugated Gt a Rabbit IgG [H&L] (code 611 132 122) for 30 min at room temperature.?? LICOR's OdysseyR Infrared Imaging System was used to scan and process the image.? Other detection systems will yield similar results.
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|
メーカー |
品番 |
包装 |
RKL
|
600-406-381
|
100 UG
|
※表示価格について
当社在庫 |
なし
|
納期目安 |
約10日
|
法規制 |
毒
|
保存温度 |
4℃
|
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