Anti MYC Epitope Tag  

Western Blot of Biotin Conjugated Rabbit anti-Myc Epitope Tag antibody. Lane 1: 12-Tag Lysate. Lane 2: None. Load: N/A. Primary antibody: None. Secondary antibody: Biotin rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Myc Epitope Tag. Other band(s): Myc splice variants and isoforms.

Anti-Myc epitope tag polyclonal antibody detects Myc-tagged recombinant proteins by western blot.？ Polyclonal rabbit-anti-Myc epitope tag at 0.5-1.0 μg/ml was used to detect 1.0 ug of recombinant protein containing the Myc epitope tag.？ A 4-20% gradient gel was used to separate the protein by SDS-PAGE.？ The protein was transferred to nitrocellulose using standard methods.？ After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye？800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature.？？ LICOR's OdysseyR Infrared Imaging System was used to scan and process the image.？ Other detection systems will yield similar results.